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精确控制细胞行为是细胞和组织工程学研究的终目标，材料表面形貌特别是微/纳米图案化形貌对细胞行为产生重要影响。本书基于著者近年来在高分子生物材料领域的科研成果撰写而成，系统地介绍了温和的无溶剂气相光接枝用于高分子材料的新方法和该法与微流体孔道技术相结合提出的掩模光接枝的高分子表面微/纳米图案化新技术，适合化学、化工、材料、生物工程等领域科研工作者阅读使用。

1 Photografting of Acrylamide onto Poly (ethylene terephthalate)

　1.1 Summary

　1.2 Introduction

　1.3 Materials, reagents and characterization

　1.4 Grafting reactor

　1.5 Grafting with acrylamide

　1.6 Hofmann rearrangement and coupling with fluoro compound

　1.7 Characterization of modified surface

　　1.7.1 Monomer vapour concentration

　　1.7.2 XPS and contact angle

　　1.7.3 Coupling reactions

　1.8 Conclusions

　　References

2 Photografting of Maleic Anhydride onto Poly (ethylene terephthalate)

　2.1 Summary

　2.2 Introduction

　2.3 Materials and reagents

　2.4 Grafting reactor

　2.5 Characterization

　2.6 Grafting with maleic anhydride (MAH)

　2.7 Conversion of surface functional groups

　　2.7.1 Hydrolysis of PET-anhydride to PET-COOH

　　2.7.2 Conversion of carboxylic PET-COOH to acid chloride PET-COCl

　　2.7.3 Coupling of amino compounds to PET-COC

　　2.7.4 Coupling of amino compound to PET- anhydride

　　2.7.5 Coupling of thiol compounds to PET-COOH

　2.8 Cell cultivation on PET-RGD surface

　2.9 Grafting and characterization

　2.10 Coupling reactions

　2.11 Surface concentrations of ligands

　2.12 Surface grafting effect on cell behaviour

　2.13 Conclusion

　References

3 Photografting of Acrylamide onto Poly (methyl methacrylate)

　3.1 Summary

　3.2 Introduction

　3.3 Grafting and coupling

　　3.3.1 Materials and reagents

　　3.3.2 Grafting reactor

　　3.3.3 Characterization

　　3.3.4 Grafting with acrylamide

　　3.3.5 Hofmann rearrangement

　　3.3.6 Analysis of amino groups

　　3.3.7 Coupling with (polyethylene glycol)

　　3.3.8 Coupling with fluorosubstituted aldehyde

　3.4 Characterization of grafted surface

　　3.4.1 On vapour phase grafting

　　3.4.2 Characterization using ESCA and contact angle

　　3.4.3 Functionalization

　　3.4.4 Coupling reactions

　3.5 Conclusions

　References

4 Micropatterning Functional Groups on Polymer Surfaces via Masked Vapor-phase Photografting

　4.1 Summary

　4.2 Introduction

　4.3 Preparation of mask and masked grafting

　　4.3.1 Materials and reagents

　　4.3.2 Mask preparation and patterned grafting

　4.4 On solvent-free vapour-phase photografting

　4.5 Micropatterning polymer surface via masked photografting

　4.6 Conclusion

　References

5 Electron Beam-induced Graft Polymerization of

　Acrylic Acid and Immobilization of Arginine-

　glycine-aspartic Acid-containing Peptide

　onto Nanopatterned Polycaprolactone

　5.1 Summary

　5.2 Introduction

　5.3 Experimental

　　5.3.1 Material and reagents

　　5.3.2 Electron beam preirradiation

　　5.3.3 Graft polymerization

　　5.3.4 Determination of surface carboxylic groups

　　5.3.5 Immobilization of RGD peptide

　　5.3.6 Deactivation of excess disulfide groups

　　5.3.7 Determination of peptide concentration

　　5.3.8 Observation of surface topography

　　5.3.9 Cell cultivation

　5.4 Results and discussion

　　5.4.1 Graft polymerization

　　5.4.2 Peptide immobilization

　　5.4.3 Cell behavior

　5.5 Conclusions

　References

6 Facile Polyester Surface Functionalization via Hydrolysis and Cell-recognizing Peptide Attachment

　6.1 Summary

　6.2 Introduction

　6.3 Experimental

　　6.3.1 Materials and regents

　　6.3.2 Preparation of PCL membrane

　　6.3.3 Hydrolysis of PCL films

　　6.3.4 Attachment of peptide

　　6.3.5 Characterization of modified surfaces

　6.4 Results and discussion

　　6.4.1 Preparation of thin films with dip-coating method

　　6.4.2 Base-catalysed surface hydrolysis

　　6.4.3 Peptide coupling onto carboxylated surface

　6.5 Conclusion

　References

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书籍介绍

《面向生物医用的高分子表面修饰和微(英文版)》基于著者近年来在高分子生物材料领域的科研成果撰写而成，系统地介绍了温和的无溶剂气相光接枝用于高分子材料的新方法和该法与微流体孔道技术相结合提出的掩模光接枝的高分子表面微/纳米图案化新技术，适合化学、化工、材料、生物工程等领域科研工作者阅读使用。精确控制细胞行为是细胞和组织工程学研究的最终目标，材料表面形貌特别是微/纳米图案化形貌对细胞行为产生重要影响。